Tag Archives: perfusion

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NIBIB Contract: 3D Bone Marrow Mimetic

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National Institute of Biomedical Imaging and Bioengineering (NIBIB) Awards KIYATEC $225,000 to Develop 3D Platelet Bank 

Contract Funds 3D Microbioreactor Bone Marrow Mimetic for Platelet Production

GREENVILLE, SC | September 30, 2015 – KIYATEC announced today that it has been awarded a $225,000 Small Business Innovation Research (SBIR) Phase I Grant from the National Institute of Biomedical Imaging and Bioengineering (NIBIB), one of the 27 institutes and centers that comprise the National Institutes of Health (NIH).  Over the course of the six month award, KIYATEC will develop 3D microbioreactors useful for the in vitro production of platelets and advance its technology around bone marrow models.  Bone marrow has important implications for the company’s focus in 3D cancer assays, especially with respect to immuno-oncology models combining cancer and the human immune system.  In collaboration with Tufts University’s biomaterial scaffold design and fabrication expertise, KIYATEC will develop a perfusion bioreactor system containing a live bone marrow mimetic capable of generating clinically relevant quantities of platelets from initial bone marrow precursor cells and then characterize their morphology and function.

3D Bone Marrow Mimetic

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KIYATEC 3DKUBE® Featured in Tufts Publication

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The 3DKUBE® perfusion bioreactor from KIYATEC was recently featured in the article entitled “Long term perfusion system supporting adipogenesis”

GREENVILLE, SC | April 2, 2015 – KIYATEC Inc. is pleased to announce that our 3DKUBE® perfusion bioreactor system was used to enable long term, in vitro viability and adipogenesis by researchers at Tufts University.  The article highlights the key advantages of 3D perfusion cell culture versus standard 2D and perfused 2D cell culture methods in creating adipogenic cells from preadipocyte cultures.

The work was authored by Rosalyn D. Abbott et al and results from many years of research into 3D perfusion cell culture techniques in the labs of David Kaplan at Tufts University.  The article may be purchased online at: http://www.sciencedirect.com/science/article/pii/S1046202315001395

Key highlights of the research include:

• 2D and 3D perfusion culture techniques supported adipogenesis of preadipocytes.
• 3D perfusion contained adipocytes that were lost in the 2D perfused cultures.
• 3D perfusion stimulated greater lipogenesis and lipolysis compared to 2D perfusion.
• 3D perfusion stimulated decreased amounts of LDH compared to 2D perfusion.
• Greater glycerol was secreted with perfusion versus static cultures.